Commercial and Therapeutic Potential of Plant-Based Fatty Acids

This chapter reviews plant-based fatty acids as well as their methods of production, applications in the industry, and benefits in treatments of cardiovascular and cerebral diseases, besides being a source of food. The fatty acids obtained from vegetable matrices have been acting as alternatives to the use of lipids of animal origin, due to their limitation in relation to the increase in demand. Thus, plants have been investigated in order to act as sources of fatty acids and assist in the supply of such demands. Vegetable oils represent not only an economical alternative but also a beneficial source of human health.


Introduction
Lipid components, especially fatty acids, are present in the most diverse forms of life, playing important roles in the structure of cell membranes and metabolic processes. In humans, omega-series fatty acids are required to maintain cell membranes, brain functions, and the transmission of nerve impulses under normal conditions. These fatty acids also play a key role in the processes of transfer of atmospheric oxygen to blood plasma, hemoglobin synthesis, and cell division. They are called essential because the human body does not synthesize them [1].
Fatty acids are classified according to the presence of double bonds between the carbon chains. They are called saturated fatty acids (SFA) if there are no double bonds; monounsaturated fatty acids (MUFA) if there is one double bond; and polyunsaturated fatty acids (PUFA) if two or more double bonds are present. Regarding the size of the carbon chain, PUFAs have number of carbons ≥ 16 and are also called long-chain polyunsaturated fatty acids, whereas those with number of carbons ≥ 20 are referred 16 Moringa oleifera Lam.
One way to minimize the problems caused by the limitations in fish oil production is to use alternative sources such as vegetable oils. Lipids found in plants present, in their composition, polyunsaturated fatty acids (PUFA), mainly omega-6 and omega-3, which are derived from linoleic acid and α-linolenic acids, respectively. Both are synthesized by plants and not by animal tissues and are classified as essential for good health and disease prevention [11].
Over the years, lipids derived from plants have been gaining prominence in the biotechnology area, mainly for the development of products with pharmacological potential [12].
When evaluating a potential source of fatty acids, its sustainability and ability to meet any demand must be considered. The possibility of a scalable production based on agriculture, coupled with low costs and ease of production, highlights the potential of plants as sources of fatty acids for human diet. Table 1 presents some plant matrices evaluated as sources of fatty acids.

Obtaining and commercial applications of fatty acids
Fatty acids present many applications, due to their physical, biological, and alimentary properties. Regarding their obtaining, there is a great diversity of conventional and modern techniques [35]. According to [36], one of the most traditional methodologies for obtaining oils rich in fatty acids, which involve the use of organic solvents, is Soxhlet [36,37]. Another technique used is cold pressing, even though it is a millenarian technique [38]. More sophisticated procedures, such as ultrasoundassisted extraction, supercritical fluids extraction, and enzyme extraction are also used to obtain such oils [39]. The extraction of oils can be carried out by Soxhlet using different parameters and organic solvents [40]. The most used solvent in this methodology is petroleum ether, due to its high solvation capacity, inertness, and good stability with oils. Time, temperature, and number of cycles are parameters that directly influence the oil yield obtained in the process [41]. In the methodology of [42], the classic extraction protocol based on chloroform and methanol is used, and it is performed in two different steps: the first one with homogenization and filtration and the second one with washing of the filtrate obtained in the first step, in order to collect the clean oil [42].
The methodology of [37] is an adaptation of that performed by [43], developed due to the need of a faster and simpler process, maintaining its efficiency and reproducibility. According to the needs faced by researchers, several adaptations based on these methodologies have already been developed, most of them with the purpose of obtaining oil of different matrices, with use of less aggressive solvents [40,43].
A technique of low environmental impact is the extraction by cold pressing, which in addition to being faster, when compared with other techniques, and of low cost, no solvent is used. However, it does not have the ability to completely remove the lipid fraction of the matrix, and due to the absence of selectivity, an extract with low degree of purity is obtained; that is, it contains a greater variety of compounds besides the fatty acids. This type of oil is widely used in food industries [44].
Among the most modern techniques, the extraction by supercritical fluid outstands, which does not use toxic organic solvents, is efficient in obtaining oils rich in fatty acids and is a process with high selectivity [44]. The most commonly used solvent is carbon dioxide, because it is nontoxic, inert, and has low critical properties. In this process, the solvent is pressurized until it becomes a fluid in the supercritical state, with characteristics of liquid and gas, and presents high solubilization and diffusion power, which is able to extract the lipid fraction [45]. The ultrasonic scanning, on the other hand, occurs through ultrasonic waves that create bubbles in the solvent used; these bubbles rupture near the cell walls causing their rupture, and consequently leading to the release of the lipid fraction [46].
In addition to these techniques, there is the microwave-assisted enzymatic extraction, in which some enzymes (cellulase, pectinase, and proteinase), due to their potential of oil release from within membranes, come into contact with the aqueous matrix and react under agitation and microwave resonance. It is a clean methodology because it does not use organic solvents, and it is possible to obtain results similar to the traditional techniques regarding the content of fatty acids [47].
With a wide range of methodologies for obtaining oils rich in fatty acids, which go beyond those mentioned in this chapter, it is possible to obtain oils of the most varied qualities for the most varied applications. The fatty acids are high-value compounds used in the food and pharmaceutical areas. They are marketed predominantly in the form of edible oils, supplements in the form of gelatinous tablets, intravenous emulsions, and in oil-based products of topical use.
Edible vegetable oils rich in fatty acids are products widely consumed worldwide, and also the main sources of omega-9 (oleic acid). Omega-9 is the most consumed fatty acid in America through its main marketed sources, such as olive oil, oleaginous fruits (almonds and nuts), grape seeds, canola, sesame, sunflower, soybean, coconut, and palm oils, among others. Most of these products are linked to a healthy lifestyle of their consumers [48].
However, the increased incidence of diseases related to the lack of a balanced diet and the presence of sedentary lifestyles is one of the main factors driving the global market for fatty acid supplements, which directly increases the demand for isolated omega supplements (3, 6, and omega-9), or their main plant sources. Such products are indicated as a source of lipids to meet the energetic needs of patients who require Commercial and Therapeutic Potential of Plant-Based Fatty Acids DOI: http://dx.doi.org/10.5772/intechopen.81122 parenteral nutrition when oral or enteral feeding is impossible, insufficient, or contraindicated [49]. It is also indicated in the treatment of rheumatoid arthritis [50].
Omega-3 supplements are generally consumed in the form of gelatinous tablets due to their residual taste, caused by their high instability to oxidation, which causes the product to exhibit odor and taste of fish. One of its main plant sources is linseed oil. These products are indicated for the prevention/treatment of cardiovascular diseases; for the reduction of triglycerides rates, total cholesterol and arterial pressure, and also in neurological treatments, improving concentration, memory, motivation, and motor abilities, besides neutralizing the stress and preventing degenerative brain diseases [51,52]. They are also indicated during pregnancy, reducing the risk of postpartum depression and mood swings, as well as improving health after child's birth [53].
Omega-6 supplements are usually marketed as evening primrose oil (EPO), which is its most popular form, being indicated in the prevention/treatment of problems related to premenstrual syndrome, diabetes, cardiovascular diseases, inflammation, skin problems, and cancer, as well as assisting the attention deficit/ hyperactivity disorder, reducing arterial hypertension and osteoporosis [54]. On the other hand, omega-9 supplements are the most commercialized in the form of intravenous emulsion. They are mostly made from refined olive and soybean oils, and found in the market in tablet form. Omega-9 supplementation is indicated for the reduction of waist circumference, combating total and bad cholesterol (LDL), and increasing the good one (HDL). Also, it presents anti-inflammatory activity and is involved in the prevention of coronary diseases, cancer, and aging [55].
Another way of commercializing fatty acids is in topical products. These products are aimed at the dermoprotection, being found in the market in liquid form, and in bandages soaked in oil. Some are indicated for nail strengthening due to their antifungal character (for example, oils derived from melaleuca, clove, thyme, and rosehip), whereas others are indicated for the treatment of all types of skin lesions, such as pressure sores, and venous stasis ulcer. Because of their emollient and healing properties, they improve the skin barrier function and reduce the symptoms of inflammation in atopic dermatitis and psoriasis, diminishing the transepidermal water loss [56,57].
These fatty acid-based products may contain one or both fatty acids plus other substances, such as vitamin A, E, and soy lecithin. They can also integrate mediumchain triglyceride formulations, linoleic and linolenic acids, mainly responsible for this therapeutic effect, since they are the main constituents of the epidermal water barrier layer. The medium-chain triglycerides present in such formulations contain predominantly caprylic, capric, caproic, and lauric acids, which can be used as a nutritional source, solvents, and product stabilizers. When present in topical products, these acids have the function of lubricating the skin and hair, making the skin more resistant to infections, protecting it from chemical and enzymatic agents, preventing dryness, and accelerating the cicatricial processes. In addition, they are free of side effects [58][59][60].
Therefore, the application of fatty acids in food and pharmaceutical areas is of fundamental importance, in order to promote the development of new natural products that offer numerous benefits to their consumers.

Nutraceutical functions
A new paradigm of food health is evolving, in which the positive aspects of diet are more emphasized. Therefore, consumers are looking for beneficial, complementary or alternative products, and the nutraceutical ones particularly stand out. Nutraceutical comes from the combination of the words "nutrition" and "pharmaceutical," which was coined in 1989 by Dr. Stephen Defelice. It is considered food or part of food, or any substance of both plant and animal origin, which has positive effects on the health, playing an important role in maintaining the normal physiological function that keeps humans healthy, including the prevention and/or treatment of diseases [61][62][63][64].
Some of the most common ways of classifying nutraceuticals can be based on food sources, mechanisms of action, chemical nature, etc. Food sources used as nutraceuticals can be categorized as dietary fiber, prebiotics, probiotics, PUFAs, antioxidant vitamins, polyphenols, and other different types of herbal foods. These nutraceutical products help fight diseases such as obesity, cardiovascular diseases, cancer, osteoporosis, arthritis, diabetes, cholesterol, among others [53,61,65].
Long-chain PUFAs are also called essential fatty acids because they are necessary for vital functions in humans, as well as being an important source of energy for most tissues. In this sense, PUFAs can be divided into two groups: omega-3 and omega-6. The major omega-3 fatty acids are α-linolenic acid (ALA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). ALA is the precursor of EPA and DHA, and its main sources are linseed, soybeans, and canola. The omega-6 fatty acids consist mainly of linoleic acid (LA), γ-linolenic acid (GLA), and arachidonic acid (ARA). LA occurs mainly in corn, soybean, and sunflower oils. Essential fatty acids should be consumed through diet, since humans do not possess the enzymes to produce them [64][65][66].
Recent studies show the importance of PUFAs as essential fatty acids, and their nutritional value in human health and disease prevention. Intake of PUFAs has been associated primarily with decreased risk of cardiovascular diseases and normal brain development [66][67][68]. They also present anti-inflammatory effects and contribute to the good functioning of vision. In the study carried out by [69], it was shown that among PUFAs, ALA can serve as a great anti-inflammatory agent of the ocular surface. Its anti-inflammatory effects are comparable to those of corticosteroids. ALA's inhibition of pro-inflammatory cytokines was associated with a significant reduction of I-κBα. According to [70], dietary supplementation with PUFAs is a promising therapeutic option for patients with rheumatoid arthritis, considered as a chronic disease, in which many inflammatory pathways contribute to structural damage, joint swelling, and systemic inflammation.
More recently, studies have shown the benefits of PUFAs in cancer prevention. According to [71], PUFAs exert inhibitory effects on the growth of colon cancer cells. Metabolites of PUFAs such as prostaglandins and leukotrienes play an important role in colon cancer. Also, they are reported in the treatment of diabetes mellitus [72], based on clinical intervention studies performed in diabetic patients. It corroborates that dietary supplementation with 0.42-5.2 g of PUFAs per day for 8 weeks might become an alternative treatment against type 2 diabetes mellitus.
PUFAs have also been attributed to anticoagulant, vasodilator, and antiaggregant activities [66,67]. Specifically, the biological activities of essential fatty acids (ALA and LA) influence on the functions and responsiveness of cell membranes, tissue metabolism, hormone signals, and others. The beneficial effects of PUFAs can be mediated by different mechanisms, including altering or regulating cell membrane structures, regulating intracellular signaling pathways, modulating gene transcription, and regulating the production of bioactive lipid mediators or production of eicosanoids (prostaglandins, leukotrienes, and thromboxanes) [68,73].
Cardiovascular diseases have been reported as the leading cause of death in the western countries. According to data obtained in 2017 by the World Health Organization, cardiovascular diseases cause approximately 31% of deaths worldwide, making it clear that prevention is important for reducing these numbers [76].
Currently, the number of deaths due to cardiovascular diseases is increasing, and this is an important reason to carry out studies in order to obtain products that can contribute to a healthier diet through the intake of substances beneficial to heart health, such as unsaturated fatty acids (omega series), as well as reduce the intake of saturated fatty acids [76][77][78][79][80][81][82].
Researchers have investigated the reduction of the risk of cardiovascular diseases by implementing a diet rich in PUFAs. These studies confirm the efficiency of the intake of these acids, demonstrating a positive effect on lipid metabolism, and also emphasizing that diets rich in SFAs promote cardiovascular damage, as well as an increase in hypercholesterolemia. Nutritionists recommend that intakes of SFAs are maintained by up to 10% relative to total energy, based on dietary guidelines for prevention of cardiovascular diseases [80,[82][83][84].
Limiting the intake of SFAs becomes an important measure for the prevention of cardiac ischemia, since healthy eating habits can affect the development of diseases and reduce the risk of their occurrence in the myocardium. Thus, daily intake of PUFAs such as omega-3 and omega-6 is important. Experimental tests were carried out with people with ischemic heart failure, and the beneficial effect of PUFAs consumption was verified by the reduction of cardiovascular risk factors such as reduction of the inflammatory process in myocardial tissue, and acceleration of the healing process of the myocardium fibrous tissue [78,[83][84][85][86][87][88][89].
Coronary heart disease relates to the development of atherosclerosis, characterized by chronic inflammation of the tunica intima of large and medium-sized arteries. Such inflammation is caused by the interaction between the smooth muscle of the arterial walls and the plasma lipids, or platelets, lipoproteins, endothelium and monocytes, causing narrowing of the coronary arteries. Thus, the maintenance of a diet rich in dietary lipids acts as a therapeutic alternative in the prevention and treatment of various cardiovascular diseases such as strokes and thrombosis [90][91][92].

Effects on brain functions
The importance of fatty acids of vegetable origin in neural development, aging, and neurodegeneration has been addressed in several studies. The brain is an organ rich in phospholipids, which make up about 25% of its dry weight [93]. Some of these fatty acids participate in the structure, biochemistry, physiology, and consequently of the cerebral function, being necessary to maintain, under normal conditions, the cellular membranes, increasing their fluidity and functionality. They also aid in the nerve impulses transmission, reinforcing the importance of the adequate consumption of these lipids to benefit patients with neurological diseases [94,95].
The consumption of PFAs is related to the reduction, prevention, and nonpharmacological treatment of some neurological diseases [96,97]. Some studies show how eating habits can affect the brain development by making a comparison between the "Mediterranean diet" and the "western diet," for example. Whereas the Mediterranean diet is rich in long-chain PFAs derived from the combination of fruits, vegetables, cereals, olive oil, and other foods, the western diet is characterized by an increase in the consumption of SFAs and transfats due to the introduction of highly processed foods [98,99]. Experimental data relate these dietary components to neurological, neurodegenerative, and psychiatric disorders, since diets with high cholesterol rates increase the risk of developing such diseases, whereas diets with low saturated-fat intake reduce the risk of dementia [100][101][102], confirming the important role of diet in pathological mechanisms related to the brain.
The hypothesis that diet-induced changes affect brain circulation may be linked to changes in brain structure [103,104]. The fatty acid content may affect the production and function of dopamine and serotonin [105], since omega-series fatty acids are fundamental for the maintenance of dopaminergic function in the brain, whereas irregularities of these fatty acids can interfere in the function of the dopaminergic receptors [104]. Healthy aging of humans on a regular diet was associated with neuroprotective properties, such as increased volume of the cortex's gray matter, higher total brain volume, and less white matter hyperintensities (lesions) [106], whereas high-energy transfat diets are associated with increased brain atrophy, and reduced total brain volume and numbers of neurons [107].
Adequate dietary intake of fatty acids or their precursors is also important during the perinatal period (before and after the baby's birth), since to ensure the normal development of the brain, newborns need more lipids than adults do. They are essential for fetal growth and development, and for neurological, behavioral, and learning functions [108][109][110]. Therefore, insufficient supplementation during early life may also aid in the development of diseases related to poor brain development, such as coordination disorder, dyspraxia (neurological motor dysfunction), and attention-deficit/hyperactivity disorder [109]. And the ingestion of fatty acids, mainly of the ω-3 type, positively affects the functioning and development throughout life, increasing cognitive functionality, such as learning, memory, and attention [110,111].

Conclusion
Vegetable oils rich in essential fatty acids have been consumed instead of animal oils due to their high potential of production scalability, and because the fish oil market has not been able to satisfactorily meet the current consumers' demands. Several commercial applications have been developed with wide acceptability, and studies aimed at treating cardiovascular diseases and improving brain functions have reached promising results. In this sense, the use of plants as sources of fatty acids presents itself as a potential alternative not only in the economic scope, but also for human health.
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